Study of in vitro culture of Gymnostachyum species
Tin Tin Khaing, A.L.T Perera[1], V.A Sumanasinghe2, D.S.A Wijesundara3
Postgraduate Institute of Agriculture
University of Peradeniya
Peradeniya, Sri Lanka
ABSTRACT
Postgraduate Institute of Agriculture
University of Peradeniya
Peradeniya, Sri Lanka
ABSTRACT
The genus Gymnostachyum (Acanthaceae) consists of beautiful flowering shrubs and herbs, native to Sri Lanka. Gymnostachyum zeylanicum can be used as an ornamental plant and a ground cover in landscape designing. The present research was conducted to find the optimum in vitro conditions for rapid multiplication of novel Gymnostachyum plants developed through mutation. Modified MS liquid and solid media consisting of MS salts + 0.1g/l myoinositol without hormones and modified with BAP + NAA, supplemented by 20g/l sucrose media at pH -5.7 were used as treatments in the in-vitro cultures.
The MS medium containing 5mg/lit BAP + 1mg/lit NAA gave the best results for callus regeneration as well as plantlet formation. Explants of leaf tissue and immature seed cultures showed high potential for regeneration under in vitro conditions. Callus formation and regeneration of the plantlets began about six weeks after culturing of leaf explants. The plantlets formed by leaf explants produced without roots and the plantlets from immature embryos developed roots.
The MS medium containing 5mg/lit BAP + 1mg/lit NAA gave the best results for callus regeneration as well as plantlet formation. Explants of leaf tissue and immature seed cultures showed high potential for regeneration under in vitro conditions. Callus formation and regeneration of the plantlets began about six weeks after culturing of leaf explants. The plantlets formed by leaf explants produced without roots and the plantlets from immature embryos developed roots.
Key words: Gymnostachyum, MS medium, explants,
If you want to have full paper, please contact to this address; tintinkhaing@gmail.com
+of+Paddy1.jpg)
